You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
yabJ [2017-08-16 10:02:11]
2-iminobutanoate/2-iminopropanoate deaminase, biosynthesis of isoleucine
function
biosynthesis of isoleucine
product
2-iminobutanoate/2-iminopropanoate deaminase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids][category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]Gene
Coordinates
55,295 → 55,672
The protein
Catalyzed reaction/ biological activity
2-iminobutanoate + H2O = 2-oxobutanoate + NH3 [pubmed|22094463]2-iminopropanoate + H2O = pyruvate + NH3 [pubmed|22094463]Protein family
Ref.3 (according to Swiss-Prot) YjgF familyStructure
[PDB|1QD9], 1QD9 [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=11484 NCBI] [Pubmed|10557275][SW|Localization]
cytoplasm (according to Swiss-Prot)additional information
was originally thought to be required for activity of [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR], but this is due to the orientation of the marker gene downstream of [gene|search|purR ]in the [gene|search|yabJ ]mutant strain used in [pubmed|10368157]Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, [Pubmed|7638212], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]regulation
negative autoregulation [Pubmed|7638212]view in new tabBiological materials
Mutant
MGNA-B911 (yabJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1910 NBRP B. subtilis, Japan]References
10368157,7638212,7638212,10557275,22094463